Review



human holo transferrin  (Merck & Co)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 86
      Buy from Supplier

    Structured Review

    Merck & Co human holo transferrin
    Human Holo Transferrin, supplied by Merck & Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/holo-transferrin/transferrin/pmc12996415-390-0-2
    Average 86 stars, based on 1 article reviews
    human holo transferrin - by Bioz Stars, 2026-09
    86/100 stars

    Images

    Related Articles

    Cell Culture:

    Article Title: Loss of KIF13B causes time-dependent changes in ciliary polycystin-2 levels and extracellular vesicle release
    Article Snippet: mCCD cells were grown at 37 °C with 5% humidified CO 2 in DMEM/F12 with GlutaMaX (Thermo Fisher Scientific, cat. #10565018) supplemented with 2% fetal bovine serum (FBS, heat inactivated, Merck), 5 μg/ml insulin (Merck, cat. # I6634), 5 μg/ml holo-transferrin (Merck, cat. #T0665), 5 ng/ml EGF (Merck, cat. #SRP3196), 50 nM dexamethasone (Merck, cat. #D4902), 60 nM sodium selenite (Merck, cat. #S5261), 1 nM 3,3′,5-Triiodo-L-thyronine sodium salt (Merck, cat. #T6397), and 1% penicillin-streptomycin (Merck, cat. #P0781).

    Article Title: Proteomic analysis reveals a potential role for extracellular vesicles within the erythroblastic island niche
    Article Snippet: At day 8 cells were centrifuged and cultured until day 10 in filtered and unfiltered macrophage condition media in the presence of cytokine cocktail B (60 ng/mL of SCF, 3U/mL of EPO, 1uM of Hydrocortisone (Stemcell Technologies) and 300 μg/mL of holo-transferrin (Merck)).

    Article Title: Proteomic analysis reveals a potential role for extracellular vesicles within the erythroblastic island niche
    Article Snippet: Briefly CD34 + cells were first expanded for 6 days in ISHI media [IMDM (Gibco), 3U/mL Heparin (Merck), 10 μg/mL Insulin (Sigma), 200 μg/mL of holo-transferrin (Merck) and 5% Human AB serum (Sigma)) in the presence of cytokine cocktail A (60 ng/mL of SCF (Life Technologies), 5 ng/mL of IL3 (Peprotech) and 3U/mL of EPO (R&D Bio-Techne)].

    Article Title: The HASTER lncRNA promoter is a cis -acting transcriptional stabilizer of HNF1A
    Article Snippet: Cells were seeded at 300,000 cells per 24-well plate in 10 μM Y27632 mTeSR1 and differentiation was started after 24 h. The following media were used for differentiation: (1) S1 medium was prepared with MCDB 131 Medium (10372019; Thermo Fisher Scientific) supplemented with 8 mM d -(+)-Glucose (G7528; Merck), 2.46 g l −1 NaHCO 3 (S3817; Merck), 2% BSA Fraction V (10735078001; Roche), 1:50,000 Insulin-Transferrin-Selenium-Ethanolamine (ITS-X) (51500056; Thermo Fisher Scientific), 2 mM GlutaMAX (35050061; Thermo Fisher Scientific) and 0.25 mM l -ascorbic acid (A4544; Merck); (2) RPMI/B27 medium was prepared with RPMI 1640 Medium, GlutaMAX Supplement (61870010; Thermo Fisher Scientific) supplemented with B-27 Supplement (17504044; Thermo Fisher Scientific) and MEM Non-Essential Amino Acids Solution (11140035; Thermo Fisher Scientific); and (3) hepatocyte growth medium (HGM) was prepared with HBM Basal Medium (CC-3199; Lonza) supplemented with 3.75 g ml −1 BSA Fraction V, 250 μg ml −1 l -ascorbic acid, 10 μg ml −1 holo-Transferrin (T0665; Merck), 0.5 μg ml −1 Hydrocortisone (H0888; Merck), 5 μg ml −1 human Insulin and 10 ng ml −1 epidermal growth factor (236-EG-200; R&D Systems).

    Article Title: eIF6 rebinding dynamically couples ribosome maturation and translation
    Article Snippet: Briefly, 1-2.5 × 10 5 CFU-E/proerythroblasts isolated from Dox-treated mice were seeded on fibronectin-coated (2 μg/mL; Merck) 48-well plates in Iscove’s modified Dulbecco’s medium (IMDM; Thermo Fisher Scientific) containing FCS (15 %), bovine serum albumin (BSA; 1 %; Stem Cell Technologies), mSCF (10 ng/mL), recombinant human erythropoietin (10 U/mL; Cell Signaling Technology), human recombinant insulin (100 μg/mL; Merck), recombinant human insulin-like growth factor 1 (hIGF1; 100 ng/mL; Thermo Fisher Scientific), holo-transferrin (200 μg/mL; Merck), L-glutamine (2 mM; Merck), β-mercaptoethanol (50 μM) and P/S.

    Article Title: eIF6 rebinding dynamically couples ribosome maturation and translation
    Article Snippet: Briefly, 1–2.5 × 10 5 CFU-E/proerythroblasts isolated from Dox-treated mice were seeded on fibronectin-coated (2 μg/mL; Merck) 48-well plates in Iscove’s modified Dulbecco’s medium (IMDM; Thermo Fisher Scientific) containing FCS (15%), bovine serum albumin (BSA; 1%; Stem Cell Technologies), mSCF (10 ng/mL), recombinant human erythropoietin (10 U/mL; Cell Signaling Technology), human recombinant insulin (100 μg/mL; Merck), recombinant human insulin-like growth factor 1 (hIGF1; 100 ng/mL; Thermo Fisher Scientific), holo-transferrin (200 μg/mL; Merck), L-glutamine (2 mM; Merck), β-mercaptoethanol (50 μM) and P/S.

    Isolation:

    Article Title: Loss of KIF13B causes time-dependent changes in ciliary polycystin-2 levels and extracellular vesicle release
    Article Snippet: mCCD cells were grown at 37 °C with 5% humidified CO 2 in DMEM/F12 with GlutaMaX (Thermo Fisher Scientific, cat. #10565018) supplemented with 2% fetal bovine serum (FBS, heat inactivated, Merck), 5 μg/ml insulin (Merck, cat. # I6634), 5 μg/ml holo-transferrin (Merck, cat. #T0665), 5 ng/ml EGF (Merck, cat. #SRP3196), 50 nM dexamethasone (Merck, cat. #D4902), 60 nM sodium selenite (Merck, cat. #S5261), 1 nM 3,3′,5-Triiodo-L-thyronine sodium salt (Merck, cat. #T6397), and 1% penicillin-streptomycin (Merck, cat. #P0781).

    Article Title: Proteomic analysis reveals a potential role for extracellular vesicles within the erythroblastic island niche
    Article Snippet: At day 8 cells were centrifuged and cultured until day 10 in filtered and unfiltered macrophage condition media in the presence of cytokine cocktail B (60 ng/mL of SCF, 3U/mL of EPO, 1uM of Hydrocortisone (Stemcell Technologies) and 300 μg/mL of holo-transferrin (Merck)).

    Article Title: Proteomic analysis reveals a potential role for extracellular vesicles within the erythroblastic island niche
    Article Snippet: Briefly CD34 + cells were first expanded for 6 days in ISHI media [IMDM (Gibco), 3U/mL Heparin (Merck), 10 μg/mL Insulin (Sigma), 200 μg/mL of holo-transferrin (Merck) and 5% Human AB serum (Sigma)) in the presence of cytokine cocktail A (60 ng/mL of SCF (Life Technologies), 5 ng/mL of IL3 (Peprotech) and 3U/mL of EPO (R&D Bio-Techne)].

    Article Title: The HASTER lncRNA promoter is a cis -acting transcriptional stabilizer of HNF1A
    Article Snippet: Cells were seeded at 300,000 cells per 24-well plate in 10 μM Y27632 mTeSR1 and differentiation was started after 24 h. The following media were used for differentiation: (1) S1 medium was prepared with MCDB 131 Medium (10372019; Thermo Fisher Scientific) supplemented with 8 mM d -(+)-Glucose (G7528; Merck), 2.46 g l −1 NaHCO 3 (S3817; Merck), 2% BSA Fraction V (10735078001; Roche), 1:50,000 Insulin-Transferrin-Selenium-Ethanolamine (ITS-X) (51500056; Thermo Fisher Scientific), 2 mM GlutaMAX (35050061; Thermo Fisher Scientific) and 0.25 mM l -ascorbic acid (A4544; Merck); (2) RPMI/B27 medium was prepared with RPMI 1640 Medium, GlutaMAX Supplement (61870010; Thermo Fisher Scientific) supplemented with B-27 Supplement (17504044; Thermo Fisher Scientific) and MEM Non-Essential Amino Acids Solution (11140035; Thermo Fisher Scientific); and (3) hepatocyte growth medium (HGM) was prepared with HBM Basal Medium (CC-3199; Lonza) supplemented with 3.75 g ml −1 BSA Fraction V, 250 μg ml −1 l -ascorbic acid, 10 μg ml −1 holo-Transferrin (T0665; Merck), 0.5 μg ml −1 Hydrocortisone (H0888; Merck), 5 μg ml −1 human Insulin and 10 ng ml −1 epidermal growth factor (236-EG-200; R&D Systems).

    Article Title: eIF6 rebinding dynamically couples ribosome maturation and translation
    Article Snippet: Briefly, 1-2.5 × 10 5 CFU-E/proerythroblasts isolated from Dox-treated mice were seeded on fibronectin-coated (2 μg/mL; Merck) 48-well plates in Iscove’s modified Dulbecco’s medium (IMDM; Thermo Fisher Scientific) containing FCS (15 %), bovine serum albumin (BSA; 1 %; Stem Cell Technologies), mSCF (10 ng/mL), recombinant human erythropoietin (10 U/mL; Cell Signaling Technology), human recombinant insulin (100 μg/mL; Merck), recombinant human insulin-like growth factor 1 (hIGF1; 100 ng/mL; Thermo Fisher Scientific), holo-transferrin (200 μg/mL; Merck), L-glutamine (2 mM; Merck), β-mercaptoethanol (50 μM) and P/S.

    Article Title: eIF6 rebinding dynamically couples ribosome maturation and translation
    Article Snippet: Briefly, 1–2.5 × 10 5 CFU-E/proerythroblasts isolated from Dox-treated mice were seeded on fibronectin-coated (2 μg/mL; Merck) 48-well plates in Iscove’s modified Dulbecco’s medium (IMDM; Thermo Fisher Scientific) containing FCS (15%), bovine serum albumin (BSA; 1%; Stem Cell Technologies), mSCF (10 ng/mL), recombinant human erythropoietin (10 U/mL; Cell Signaling Technology), human recombinant insulin (100 μg/mL; Merck), recombinant human insulin-like growth factor 1 (hIGF1; 100 ng/mL; Thermo Fisher Scientific), holo-transferrin (200 μg/mL; Merck), L-glutamine (2 mM; Merck), β-mercaptoethanol (50 μM) and P/S.

    Modification:

    Article Title: Loss of KIF13B causes time-dependent changes in ciliary polycystin-2 levels and extracellular vesicle release
    Article Snippet: mCCD cells were grown at 37 °C with 5% humidified CO 2 in DMEM/F12 with GlutaMaX (Thermo Fisher Scientific, cat. #10565018) supplemented with 2% fetal bovine serum (FBS, heat inactivated, Merck), 5 μg/ml insulin (Merck, cat. # I6634), 5 μg/ml holo-transferrin (Merck, cat. #T0665), 5 ng/ml EGF (Merck, cat. #SRP3196), 50 nM dexamethasone (Merck, cat. #D4902), 60 nM sodium selenite (Merck, cat. #S5261), 1 nM 3,3′,5-Triiodo-L-thyronine sodium salt (Merck, cat. #T6397), and 1% penicillin-streptomycin (Merck, cat. #P0781).

    Article Title: Proteomic analysis reveals a potential role for extracellular vesicles within the erythroblastic island niche
    Article Snippet: At day 8 cells were centrifuged and cultured until day 10 in filtered and unfiltered macrophage condition media in the presence of cytokine cocktail B (60 ng/mL of SCF, 3U/mL of EPO, 1uM of Hydrocortisone (Stemcell Technologies) and 300 μg/mL of holo-transferrin (Merck)).

    Article Title: Proteomic analysis reveals a potential role for extracellular vesicles within the erythroblastic island niche
    Article Snippet: Briefly CD34 + cells were first expanded for 6 days in ISHI media [IMDM (Gibco), 3U/mL Heparin (Merck), 10 μg/mL Insulin (Sigma), 200 μg/mL of holo-transferrin (Merck) and 5% Human AB serum (Sigma)) in the presence of cytokine cocktail A (60 ng/mL of SCF (Life Technologies), 5 ng/mL of IL3 (Peprotech) and 3U/mL of EPO (R&D Bio-Techne)].

    Article Title: The HASTER lncRNA promoter is a cis -acting transcriptional stabilizer of HNF1A
    Article Snippet: Cells were seeded at 300,000 cells per 24-well plate in 10 μM Y27632 mTeSR1 and differentiation was started after 24 h. The following media were used for differentiation: (1) S1 medium was prepared with MCDB 131 Medium (10372019; Thermo Fisher Scientific) supplemented with 8 mM d -(+)-Glucose (G7528; Merck), 2.46 g l −1 NaHCO 3 (S3817; Merck), 2% BSA Fraction V (10735078001; Roche), 1:50,000 Insulin-Transferrin-Selenium-Ethanolamine (ITS-X) (51500056; Thermo Fisher Scientific), 2 mM GlutaMAX (35050061; Thermo Fisher Scientific) and 0.25 mM l -ascorbic acid (A4544; Merck); (2) RPMI/B27 medium was prepared with RPMI 1640 Medium, GlutaMAX Supplement (61870010; Thermo Fisher Scientific) supplemented with B-27 Supplement (17504044; Thermo Fisher Scientific) and MEM Non-Essential Amino Acids Solution (11140035; Thermo Fisher Scientific); and (3) hepatocyte growth medium (HGM) was prepared with HBM Basal Medium (CC-3199; Lonza) supplemented with 3.75 g ml −1 BSA Fraction V, 250 μg ml −1 l -ascorbic acid, 10 μg ml −1 holo-Transferrin (T0665; Merck), 0.5 μg ml −1 Hydrocortisone (H0888; Merck), 5 μg ml −1 human Insulin and 10 ng ml −1 epidermal growth factor (236-EG-200; R&D Systems).

    Article Title: eIF6 rebinding dynamically couples ribosome maturation and translation
    Article Snippet: Briefly, 1-2.5 × 10 5 CFU-E/proerythroblasts isolated from Dox-treated mice were seeded on fibronectin-coated (2 μg/mL; Merck) 48-well plates in Iscove’s modified Dulbecco’s medium (IMDM; Thermo Fisher Scientific) containing FCS (15 %), bovine serum albumin (BSA; 1 %; Stem Cell Technologies), mSCF (10 ng/mL), recombinant human erythropoietin (10 U/mL; Cell Signaling Technology), human recombinant insulin (100 μg/mL; Merck), recombinant human insulin-like growth factor 1 (hIGF1; 100 ng/mL; Thermo Fisher Scientific), holo-transferrin (200 μg/mL; Merck), L-glutamine (2 mM; Merck), β-mercaptoethanol (50 μM) and P/S.

    Article Title: eIF6 rebinding dynamically couples ribosome maturation and translation
    Article Snippet: Briefly, 1–2.5 × 10 5 CFU-E/proerythroblasts isolated from Dox-treated mice were seeded on fibronectin-coated (2 μg/mL; Merck) 48-well plates in Iscove’s modified Dulbecco’s medium (IMDM; Thermo Fisher Scientific) containing FCS (15%), bovine serum albumin (BSA; 1%; Stem Cell Technologies), mSCF (10 ng/mL), recombinant human erythropoietin (10 U/mL; Cell Signaling Technology), human recombinant insulin (100 μg/mL; Merck), recombinant human insulin-like growth factor 1 (hIGF1; 100 ng/mL; Thermo Fisher Scientific), holo-transferrin (200 μg/mL; Merck), L-glutamine (2 mM; Merck), β-mercaptoethanol (50 μM) and P/S.

    Recombinant:

    Article Title: Loss of KIF13B causes time-dependent changes in ciliary polycystin-2 levels and extracellular vesicle release
    Article Snippet: mCCD cells were grown at 37 °C with 5% humidified CO 2 in DMEM/F12 with GlutaMaX (Thermo Fisher Scientific, cat. #10565018) supplemented with 2% fetal bovine serum (FBS, heat inactivated, Merck), 5 μg/ml insulin (Merck, cat. # I6634), 5 μg/ml holo-transferrin (Merck, cat. #T0665), 5 ng/ml EGF (Merck, cat. #SRP3196), 50 nM dexamethasone (Merck, cat. #D4902), 60 nM sodium selenite (Merck, cat. #S5261), 1 nM 3,3′,5-Triiodo-L-thyronine sodium salt (Merck, cat. #T6397), and 1% penicillin-streptomycin (Merck, cat. #P0781).

    Article Title: Proteomic analysis reveals a potential role for extracellular vesicles within the erythroblastic island niche
    Article Snippet: At day 8 cells were centrifuged and cultured until day 10 in filtered and unfiltered macrophage condition media in the presence of cytokine cocktail B (60 ng/mL of SCF, 3U/mL of EPO, 1uM of Hydrocortisone (Stemcell Technologies) and 300 μg/mL of holo-transferrin (Merck)).

    Article Title: Proteomic analysis reveals a potential role for extracellular vesicles within the erythroblastic island niche
    Article Snippet: Briefly CD34 + cells were first expanded for 6 days in ISHI media [IMDM (Gibco), 3U/mL Heparin (Merck), 10 μg/mL Insulin (Sigma), 200 μg/mL of holo-transferrin (Merck) and 5% Human AB serum (Sigma)) in the presence of cytokine cocktail A (60 ng/mL of SCF (Life Technologies), 5 ng/mL of IL3 (Peprotech) and 3U/mL of EPO (R&D Bio-Techne)].

    Article Title: The HASTER lncRNA promoter is a cis -acting transcriptional stabilizer of HNF1A
    Article Snippet: Cells were seeded at 300,000 cells per 24-well plate in 10 μM Y27632 mTeSR1 and differentiation was started after 24 h. The following media were used for differentiation: (1) S1 medium was prepared with MCDB 131 Medium (10372019; Thermo Fisher Scientific) supplemented with 8 mM d -(+)-Glucose (G7528; Merck), 2.46 g l −1 NaHCO 3 (S3817; Merck), 2% BSA Fraction V (10735078001; Roche), 1:50,000 Insulin-Transferrin-Selenium-Ethanolamine (ITS-X) (51500056; Thermo Fisher Scientific), 2 mM GlutaMAX (35050061; Thermo Fisher Scientific) and 0.25 mM l -ascorbic acid (A4544; Merck); (2) RPMI/B27 medium was prepared with RPMI 1640 Medium, GlutaMAX Supplement (61870010; Thermo Fisher Scientific) supplemented with B-27 Supplement (17504044; Thermo Fisher Scientific) and MEM Non-Essential Amino Acids Solution (11140035; Thermo Fisher Scientific); and (3) hepatocyte growth medium (HGM) was prepared with HBM Basal Medium (CC-3199; Lonza) supplemented with 3.75 g ml −1 BSA Fraction V, 250 μg ml −1 l -ascorbic acid, 10 μg ml −1 holo-Transferrin (T0665; Merck), 0.5 μg ml −1 Hydrocortisone (H0888; Merck), 5 μg ml −1 human Insulin and 10 ng ml −1 epidermal growth factor (236-EG-200; R&D Systems).

    Article Title: eIF6 rebinding dynamically couples ribosome maturation and translation
    Article Snippet: Briefly, 1-2.5 × 10 5 CFU-E/proerythroblasts isolated from Dox-treated mice were seeded on fibronectin-coated (2 μg/mL; Merck) 48-well plates in Iscove’s modified Dulbecco’s medium (IMDM; Thermo Fisher Scientific) containing FCS (15 %), bovine serum albumin (BSA; 1 %; Stem Cell Technologies), mSCF (10 ng/mL), recombinant human erythropoietin (10 U/mL; Cell Signaling Technology), human recombinant insulin (100 μg/mL; Merck), recombinant human insulin-like growth factor 1 (hIGF1; 100 ng/mL; Thermo Fisher Scientific), holo-transferrin (200 μg/mL; Merck), L-glutamine (2 mM; Merck), β-mercaptoethanol (50 μM) and P/S.

    Article Title: eIF6 rebinding dynamically couples ribosome maturation and translation
    Article Snippet: Briefly, 1–2.5 × 10 5 CFU-E/proerythroblasts isolated from Dox-treated mice were seeded on fibronectin-coated (2 μg/mL; Merck) 48-well plates in Iscove’s modified Dulbecco’s medium (IMDM; Thermo Fisher Scientific) containing FCS (15%), bovine serum albumin (BSA; 1%; Stem Cell Technologies), mSCF (10 ng/mL), recombinant human erythropoietin (10 U/mL; Cell Signaling Technology), human recombinant insulin (100 μg/mL; Merck), recombinant human insulin-like growth factor 1 (hIGF1; 100 ng/mL; Thermo Fisher Scientific), holo-transferrin (200 μg/mL; Merck), L-glutamine (2 mM; Merck), β-mercaptoethanol (50 μM) and P/S.



    Similar Products

    transferrin  (Thermo Fisher)
    95
    Thermo Fisher transferrin
    Transferrin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/holo-transferrin/Transferrin+(Holo)%2C+bovine+plasma%2C+98%2B%25/us12643945-1235-30-32
    Average 95 stars, based on 1 article reviews
    transferrin - by Bioz Stars, 2026-09
    95/100 stars
    		  Buy from Supplier
    bovine holo transferrin  (Thermo Fisher)
    95
    Thermo Fisher bovine holo transferrin
    Initial binding is to the cell surface and internalisation is detected within 60 s. Cultures of single (1-GPI) or double (2-GPI) GPI-anchored TfR expressing BES7-VSG221 and BES1-VSG221 cells were supplemented with 500 nM <t>Alexa568-labelled</t> <t>holo-transferrin</t> (Tf A568 ) and incubated for 15, 60, or 600 s and fixed by addition of formaldehyde for 5 min. Following washing with PBS containing 1% bovine serum albumin cells were visualised under Zeiss Axio Imager.Z2 with ×100 objective. Images in all these experimental time points for both 1- and 2GPI TfR cells were captured with same intensity of excitation and exposure time. ( A ) Images showing the location of bound or endocytosed transferrin in representative cells in populations and at time points as indicated. Scale bar is 5 µm. Images were processed with ZEN Blue 3.4 software without any deconvolution. Larger numbers of cells are shown in . ( B ) The variation between individual cells in transferrin binding and uptake and endocytosis shown by measurements of total fluorescence intensity of Tf A568 in BES7 VSG221 1- and 2-GPI TfR cells at 15, 60, and 600 s. Measurements were obtained using Zen Blue 3.4 software and normalising the background fluorescence . Scatter plots of measurements of individual cells are shown for 1-GPI (grey) and 2-GPI (purple) TfRs. The average and standard deviation are shown for each cell line. ( C ) Tf A568 fluorescence intensity distribution across transverse sections of randomly selected cells was measured using ImageJ software. The plots are shown for both BES7 1- and 2-GPI cell lines, showing the pattern of individual cells in different colours at 60 and 600 s. ( D ), ( E ) and ( F ) same as ( A ) ( B ) and ( C ) but with BES1-VSG221 1- and 2GPI TfR expressing cells .
    Bovine Holo Transferrin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/holo-transferrin/Transferrin+(Holo)%2C+bovine+plasma%2C+98%2B%25/pmc13186567-240-0-2
    Average 95 stars, based on 1 article reviews
    bovine holo transferrin - by Bioz Stars, 2026-09
    95/100 stars
    		  Buy from Supplier
    bovine transferrin holo form  (Thermo Fisher)
    95
    Thermo Fisher bovine transferrin holo form
    Initial binding is to the cell surface and internalisation is detected within 60 s. Cultures of single (1-GPI) or double (2-GPI) GPI-anchored TfR expressing BES7-VSG221 and BES1-VSG221 cells were supplemented with 500 nM <t>Alexa568-labelled</t> <t>holo-transferrin</t> (Tf A568 ) and incubated for 15, 60, or 600 s and fixed by addition of formaldehyde for 5 min. Following washing with PBS containing 1% bovine serum albumin cells were visualised under Zeiss Axio Imager.Z2 with ×100 objective. Images in all these experimental time points for both 1- and 2GPI TfR cells were captured with same intensity of excitation and exposure time. ( A ) Images showing the location of bound or endocytosed transferrin in representative cells in populations and at time points as indicated. Scale bar is 5 µm. Images were processed with ZEN Blue 3.4 software without any deconvolution. Larger numbers of cells are shown in . ( B ) The variation between individual cells in transferrin binding and uptake and endocytosis shown by measurements of total fluorescence intensity of Tf A568 in BES7 VSG221 1- and 2-GPI TfR cells at 15, 60, and 600 s. Measurements were obtained using Zen Blue 3.4 software and normalising the background fluorescence . Scatter plots of measurements of individual cells are shown for 1-GPI (grey) and 2-GPI (purple) TfRs. The average and standard deviation are shown for each cell line. ( C ) Tf A568 fluorescence intensity distribution across transverse sections of randomly selected cells was measured using ImageJ software. The plots are shown for both BES7 1- and 2-GPI cell lines, showing the pattern of individual cells in different colours at 60 and 600 s. ( D ), ( E ) and ( F ) same as ( A ) ( B ) and ( C ) but with BES1-VSG221 1- and 2GPI TfR expressing cells .
    Bovine Transferrin Holo Form, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/holo-transferrin/Transferrin+(Holo)%2C+bovine+plasma%2C+98%2B%25/pm42134445-76-54-58
    Average 95 stars, based on 1 article reviews
    bovine transferrin holo form - by Bioz Stars, 2026-09
    95/100 stars
    		  Buy from Supplier
    transferrin supplement  (Thermo Fisher)
    95
    Thermo Fisher transferrin supplement
    Initial binding is to the cell surface and internalisation is detected within 60 s. Cultures of single (1-GPI) or double (2-GPI) GPI-anchored TfR expressing BES7-VSG221 and BES1-VSG221 cells were supplemented with 500 nM <t>Alexa568-labelled</t> <t>holo-transferrin</t> (Tf A568 ) and incubated for 15, 60, or 600 s and fixed by addition of formaldehyde for 5 min. Following washing with PBS containing 1% bovine serum albumin cells were visualised under Zeiss Axio Imager.Z2 with ×100 objective. Images in all these experimental time points for both 1- and 2GPI TfR cells were captured with same intensity of excitation and exposure time. ( A ) Images showing the location of bound or endocytosed transferrin in representative cells in populations and at time points as indicated. Scale bar is 5 µm. Images were processed with ZEN Blue 3.4 software without any deconvolution. Larger numbers of cells are shown in . ( B ) The variation between individual cells in transferrin binding and uptake and endocytosis shown by measurements of total fluorescence intensity of Tf A568 in BES7 VSG221 1- and 2-GPI TfR cells at 15, 60, and 600 s. Measurements were obtained using Zen Blue 3.4 software and normalising the background fluorescence . Scatter plots of measurements of individual cells are shown for 1-GPI (grey) and 2-GPI (purple) TfRs. The average and standard deviation are shown for each cell line. ( C ) Tf A568 fluorescence intensity distribution across transverse sections of randomly selected cells was measured using ImageJ software. The plots are shown for both BES7 1- and 2-GPI cell lines, showing the pattern of individual cells in different colours at 60 and 600 s. ( D ), ( E ) and ( F ) same as ( A ) ( B ) and ( C ) but with BES1-VSG221 1- and 2GPI TfR expressing cells .
    Transferrin Supplement, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/holo-transferrin/Transferrin+(Holo)%2C+bovine+plasma%2C+98%2B%25/bio_rxiv__64898__2026__02__06__704464-215-26-28
    Average 95 stars, based on 1 article reviews
    transferrin supplement - by Bioz Stars, 2026-09
    95/100 stars
    		  Buy from Supplier
    human holo transferrin  (Merck & Co)
    86
    Merck & Co human holo transferrin
    Initial binding is to the cell surface and internalisation is detected within 60 s. Cultures of single (1-GPI) or double (2-GPI) GPI-anchored TfR expressing BES7-VSG221 and BES1-VSG221 cells were supplemented with 500 nM <t>Alexa568-labelled</t> <t>holo-transferrin</t> (Tf A568 ) and incubated for 15, 60, or 600 s and fixed by addition of formaldehyde for 5 min. Following washing with PBS containing 1% bovine serum albumin cells were visualised under Zeiss Axio Imager.Z2 with ×100 objective. Images in all these experimental time points for both 1- and 2GPI TfR cells were captured with same intensity of excitation and exposure time. ( A ) Images showing the location of bound or endocytosed transferrin in representative cells in populations and at time points as indicated. Scale bar is 5 µm. Images were processed with ZEN Blue 3.4 software without any deconvolution. Larger numbers of cells are shown in . ( B ) The variation between individual cells in transferrin binding and uptake and endocytosis shown by measurements of total fluorescence intensity of Tf A568 in BES7 VSG221 1- and 2-GPI TfR cells at 15, 60, and 600 s. Measurements were obtained using Zen Blue 3.4 software and normalising the background fluorescence . Scatter plots of measurements of individual cells are shown for 1-GPI (grey) and 2-GPI (purple) TfRs. The average and standard deviation are shown for each cell line. ( C ) Tf A568 fluorescence intensity distribution across transverse sections of randomly selected cells was measured using ImageJ software. The plots are shown for both BES7 1- and 2-GPI cell lines, showing the pattern of individual cells in different colours at 60 and 600 s. ( D ), ( E ) and ( F ) same as ( A ) ( B ) and ( C ) but with BES1-VSG221 1- and 2GPI TfR expressing cells .
    Human Holo Transferrin, supplied by Merck & Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/holo-transferrin/transferrin/pmc12996415-390-0-2
    Average 86 stars, based on 1 article reviews
    human holo transferrin - by Bioz Stars, 2026-09
    86/100 stars
    		  Buy from Supplier

    Image Search Results


    Initial binding is to the cell surface and internalisation is detected within 60 s. Cultures of single (1-GPI) or double (2-GPI) GPI-anchored TfR expressing BES7-VSG221 and BES1-VSG221 cells were supplemented with 500 nM Alexa568-labelled holo-transferrin (Tf A568 ) and incubated for 15, 60, or 600 s and fixed by addition of formaldehyde for 5 min. Following washing with PBS containing 1% bovine serum albumin cells were visualised under Zeiss Axio Imager.Z2 with ×100 objective. Images in all these experimental time points for both 1- and 2GPI TfR cells were captured with same intensity of excitation and exposure time. ( A ) Images showing the location of bound or endocytosed transferrin in representative cells in populations and at time points as indicated. Scale bar is 5 µm. Images were processed with ZEN Blue 3.4 software without any deconvolution. Larger numbers of cells are shown in . ( B ) The variation between individual cells in transferrin binding and uptake and endocytosis shown by measurements of total fluorescence intensity of Tf A568 in BES7 VSG221 1- and 2-GPI TfR cells at 15, 60, and 600 s. Measurements were obtained using Zen Blue 3.4 software and normalising the background fluorescence . Scatter plots of measurements of individual cells are shown for 1-GPI (grey) and 2-GPI (purple) TfRs. The average and standard deviation are shown for each cell line. ( C ) Tf A568 fluorescence intensity distribution across transverse sections of randomly selected cells was measured using ImageJ software. The plots are shown for both BES7 1- and 2-GPI cell lines, showing the pattern of individual cells in different colours at 60 and 600 s. ( D ), ( E ) and ( F ) same as ( A ) ( B ) and ( C ) but with BES1-VSG221 1- and 2GPI TfR expressing cells .

    Journal: eLife

    Article Title: Cell surface localisation of GPI-anchored receptors in Trypanosoma brucei

    doi: 10.7554/eLife.107191

    Figure Lengend Snippet: Initial binding is to the cell surface and internalisation is detected within 60 s. Cultures of single (1-GPI) or double (2-GPI) GPI-anchored TfR expressing BES7-VSG221 and BES1-VSG221 cells were supplemented with 500 nM Alexa568-labelled holo-transferrin (Tf A568 ) and incubated for 15, 60, or 600 s and fixed by addition of formaldehyde for 5 min. Following washing with PBS containing 1% bovine serum albumin cells were visualised under Zeiss Axio Imager.Z2 with ×100 objective. Images in all these experimental time points for both 1- and 2GPI TfR cells were captured with same intensity of excitation and exposure time. ( A ) Images showing the location of bound or endocytosed transferrin in representative cells in populations and at time points as indicated. Scale bar is 5 µm. Images were processed with ZEN Blue 3.4 software without any deconvolution. Larger numbers of cells are shown in . ( B ) The variation between individual cells in transferrin binding and uptake and endocytosis shown by measurements of total fluorescence intensity of Tf A568 in BES7 VSG221 1- and 2-GPI TfR cells at 15, 60, and 600 s. Measurements were obtained using Zen Blue 3.4 software and normalising the background fluorescence . Scatter plots of measurements of individual cells are shown for 1-GPI (grey) and 2-GPI (purple) TfRs. The average and standard deviation are shown for each cell line. ( C ) Tf A568 fluorescence intensity distribution across transverse sections of randomly selected cells was measured using ImageJ software. The plots are shown for both BES7 1- and 2-GPI cell lines, showing the pattern of individual cells in different colours at 60 and 600 s. ( D ), ( E ) and ( F ) same as ( A ) ( B ) and ( C ) but with BES1-VSG221 1- and 2GPI TfR expressing cells .

    Article Snippet: Bovine holo-transferrin (Thermo Fisher) was dialyzed against PBS and labelled with Alexa fluor 568 NHS ester (Thermo Fisher) following the manufacturer’s protocol.

    Techniques: Binding Assay, Expressing, Incubation, Software, Fluorescence, Standard Deviation

    ( A ) Clustal W alignment of the polypeptide sequences of cow and dog transferrin. ( B ) Cultures were supplemented with 500 nM Alexa568-labelled canine holo-transferrin and incubated for 60 s and fixed in culture by addition of formaldehyde to 1%. Following washing with PBS containing 1% bovine serum albumin cells were visualised under Zeiss Axio Imager.Z2 with x100 objective. Images for both BES7 VSG221 1- and 2-GPI TfR cells were captured with same intensity of excitation and exposure time. Images showing the absence of any bound or endocytosed canine transferrin in around 40 cells in population of both 1- and 2GPI TfR cells. Scale bar is 20 µm. Images were processed ZEN Blue 3.4 software.

    Journal: eLife

    Article Title: Cell surface localisation of GPI-anchored receptors in Trypanosoma brucei

    doi: 10.7554/eLife.107191

    Figure Lengend Snippet: ( A ) Clustal W alignment of the polypeptide sequences of cow and dog transferrin. ( B ) Cultures were supplemented with 500 nM Alexa568-labelled canine holo-transferrin and incubated for 60 s and fixed in culture by addition of formaldehyde to 1%. Following washing with PBS containing 1% bovine serum albumin cells were visualised under Zeiss Axio Imager.Z2 with x100 objective. Images for both BES7 VSG221 1- and 2-GPI TfR cells were captured with same intensity of excitation and exposure time. Images showing the absence of any bound or endocytosed canine transferrin in around 40 cells in population of both 1- and 2GPI TfR cells. Scale bar is 20 µm. Images were processed ZEN Blue 3.4 software.

    Article Snippet: Bovine holo-transferrin (Thermo Fisher) was dialyzed against PBS and labelled with Alexa fluor 568 NHS ester (Thermo Fisher) following the manufacturer’s protocol.

    Techniques: Incubation, Software